Characteristics of Citrus cell cultures during undifferentiated growth on sucrose and somatic embryogenesis on galactose
Identifieur interne : 000240 ( France/Analysis ); précédent : 000239; suivant : 000241Characteristics of Citrus cell cultures during undifferentiated growth on sucrose and somatic embryogenesis on galactose
Auteurs : Cécile Cabasson [France] ; Patrick Ollitrault [France] ; Francois-Xavier Côte [France] ; Nicole Michaux-Ferriére [France] ; Dominique Dambier [France] ; Régine Dalnic [France] ; Claude Teisson [France]Source :
- Physiologia Plantarum [ 0031-9317 ] ; 1995-04.
English descriptors
- KwdEn :
Abstract
Growth of willow‐leaf mandarin (Citrus deliciosa Ten.) nucellar callus cultures without embryo formation was supported on sucrose as the sole carbon source. Somatic embryogenesis was obtained by substituting galactose for sucrose at the same concentration (0.15 M). A histocytological study revealed proliferation of young globular embryos during the first half of the 18‐day culture cycle, irrespective of the carbon source. However, during the second half of the cycle, further embryo development failed in cell cultures on sucrose‐containing medium. The rate of cell growth and the carbohydrate depletion of the medium were 3‐fold higher on sucrose than on galactose‐containing medium. Cell suspensions on sucrose differed by an increase in calcium, phosphorus, magnesium and potassium utilization soon after subculturing, followed by a sharp decrease. Finally, the phosphorus utilization rate per g dry weight formed was 2‐fold higher in cell cultures on galactose during the second half of the culture cycle, coinciding with further embryo ontogenesis.
Url:
DOI: 10.1111/j.1399-3054.1995.tb06844.x
Affiliations:
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ISTEX:E7B20A432D05345D94F072AADDBB4E4ED894BE77Le document en format XML
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Somatic embryogenesis was obtained by substituting galactose for sucrose at the same concentration (0.15 M). A histocytological study revealed proliferation of young globular embryos during the first half of the 18‐day culture cycle, irrespective of the carbon source. However, during the second half of the cycle, further embryo development failed in cell cultures on sucrose‐containing medium. The rate of cell growth and the carbohydrate depletion of the medium were 3‐fold higher on sucrose than on galactose‐containing medium. Cell suspensions on sucrose differed by an increase in calcium, phosphorus, magnesium and potassium utilization soon after subculturing, followed by a sharp decrease. Finally, the phosphorus utilization rate per g dry weight formed was 2‐fold higher in cell cultures on galactose during the second half of the culture cycle, coinciding with further embryo ontogenesis.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Cabasson, Cecile" sort="Cabasson, Cecile" uniqKey="Cabasson C" first="Cécile" last="Cabasson">Cécile Cabasson</name></region><name sortKey="Cote, Francois Avier" sort="Cote, Francois Avier" uniqKey="Cote F" first="Francois-Xavier" last="Côte">Francois-Xavier Côte</name><name sortKey="Dalnic, Regine" sort="Dalnic, Regine" uniqKey="Dalnic R" first="Régine" last="Dalnic">Régine Dalnic</name><name sortKey="Dambier, Dominique" sort="Dambier, Dominique" uniqKey="Dambier D" first="Dominique" last="Dambier">Dominique Dambier</name><name sortKey="Michaux Erriere, Nicole" sort="Michaux Erriere, Nicole" uniqKey="Michaux Erriere N" first="Nicole" last="Michaux-Ferriére">Nicole Michaux-Ferriére</name><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><name sortKey="Teisson, Claude" sort="Teisson, Claude" uniqKey="Teisson C" first="Claude" last="Teisson">Claude Teisson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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